Research | Engineered nanopores

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We continue to engineer new pores for applications in biotechnology, including sensing and sequencing, single-molecule chemistry and the fabrication of synthetic tissues. Recent examples include pores containing unnatural amino acids assembled by native chemical ligation, pores assembled from synthetic peptides, and truncated pores that nonetheless form openings in lipid bilayers. In a new venture, we have prepared DNA nanostructure-polypeptide hybrids that form large pores of defined diameter.


Research papers:

Stoddart, D., Ayub, M., Höfler, L., Raychaudhuri, P., Klingelhoefer, J.W., Maglia, G., Heron, A. and Bayley, H., Functional truncated membrane pores. Proc. Natl. Acad. Sci. USA, 111, 2425 (2014). DOI:10.1073/pnas.1312976111

Spruijt, E., Tusk, S.E. and Bayley, H., DNA scaffolds support stable and uniform peptide nanopores. Nature Nanotechnology 13, 739 (2018). DOI:10.1038/s41565-018-0139-6

Review:

Ayub, M. and Bayley, H., Engineered transmembrane pores. Curr. Op. Chem. Biol., 34, 117 (2016). DOI:10.1016/j.cbpa.2016.08.005