We are also investigating the use of protein pores for sequencing single molecules of DNA and RNA, and a spin-out company Oxford Nanopore focuses on this technology (https://www.nanoporetech.com/). A critical issue is the implementation of highly parallel analyte detection and sequencing, and we are currently attempting to develop technologies that will allow simultaneous recording from thousands of protein nanopores.
Harrington, L., Cheley, S. Alexander, L.T., Knapp, S. and Bayley, H. Stochastic detection of Pim kinases reveals electrostatically enhanced association of a peptide substrate. Proc. Natl. Acad. Sci. USA 110, E4417-E4426 (2013). www.pnas.org/cgi/doi/10.1073/pnas.1312739110
Huang, S., Romero-Ruiz, M., Castell, O.K., Bayley, H., and Wallace, M.I. High-throughput optical sensing of nucleic acids in a nanopore array. Nature Nanotechnology 10, 986-991 (2015). DOI: 10.1038/NNANO.2015.189
Bayley, H. Nanopore sequencing: from imagination to reality. Clinical Chemistry 61, 25-31 (2015). DOI: 10.1373/clinchem.2014.223016